Laboratory of Biological polymer compounds

fade

The Laboratory of Biological Polymer Compounds was founded in 2005 by merger of the Chemical Analyses Laboratory and the Laboratory of Biophysical research Methods. It is one of the Institute’s youngest departments. From 1976, the Laboratory of Biophysical research Methods headed by K. Hushcha, and the Chemical Analyses Laboratory headed by S. Veremeichenko, had been support of the Institute’s departments and laboratories in the technical implementation of their scientific ideas. The Laboratories’ main task had been systematic and high quality fulfillment of works aimed at studying of different biological objects and their features with the use of biophysical and analytical research techniques.

Currently, the Laboratory of Biological Polymer Compounds has united the equipment and possibilities of its two predecessors. The available equipment (an amino acid analyzer, gas-liquid chromatographs, atomic-adsorption spectrophotometers, ultracentrifuges, spectrophotometers, an electronic microscope, freeze drying) and the Lab staff’s experience enable to conduct various analyses and fulfill different tasks.

So, the Laboratory workers run analyses on amino acid, monosaccharides, fatty-acids composition of biological objects; determine single- and polyatomic alcohols, hydrocarbons, ethylene and acetylene. Methods on determination of the elementary composition of liquid samples of different origin are widely applied when studying the microelements’ impact upon the microorganisms’ functions and properties, spectroscopic research, lyophilization of the microbial material, hydrodynamic methods (analytic and preparative ultracentrifuging). Lately, the Laboratory of Biological Polymer Compounds has become additionally equipped with new-generation chromatographs. A modern Agilent-made gas chromatograph with mass-spectrometric detector, model 6890N/5973 inert, is equipped with a massspectral library NIST02 used to identify the compounds. The system is meant to be used in the modes of electron ionization and chemical ionization, thereby enabling to run high-quality qualitative and quantitative analyses, determine the molecular weight of the reviewed objects and identification of unknown compounds.

One of the latest technological developments in liquid chromatography, the Agilent 1200 chromatograph is equipped with diode-array, fluorescent and mass-spectrometric detectors. The available equipment enables to conduct chromatographic and chromato-mass-spectrometric research on the ultra-modern scientific level as well as to broaden the spectrum and quality of feasible research.

Nowadays, these devices are widely applied for the fulfillment of scientific research programs of the Institute’s departments and laboratories. Various analyses on content identification of microbial polymers take place at the Lab; quantitative and qualitative contents of various biologically active substances produced by microorganisms are studied.

The spectrum of the methods applied is planned to be significantly expanded at cost of research of pesticides, herbicides, phenol compounds, proteins, nucleosides, oligonucleotides, vitamins, carotenoids, flavonoids, organic acids, amino compounds, etc.